AOTF microscope for imaging with increased speed and spectral versatility
Journal Article, Biophysical Journal, Vol. 73, No. 3, pp. 1215 - 1222, September, 1997
Abstract
We have developed a new fluorescence microscope that addresses the spectral and speed limitations of current light microscopy instrumentation. In the present device, interference and neutral density filters normally used for fluorescence excitation and detection are replaced by acousto-optic tunable filters (AOTFs). Improvements are described, including the use of a dispersing prism in conjunction with the imaging AOTF and an oblique-illumination excitation scheme, which together enable the AOTF microscope to produce images comparable to those obtained with conventional fluorescence instruments.
BibTeX
@article{Wachman-1997-16409,author = {E. S. Wachman and W. Niu and Daniel Farkas},
title = {AOTF microscope for imaging with increased speed and spectral versatility},
journal = {Biophysical Journal},
year = {1997},
month = {September},
volume = {73},
number = {3},
pages = {1215 - 1222},
}
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